|
KMID : 0370220040480010013
|
|
Yakhak Hoeji
2004 Volume.48 No. 1 p.13 ~ p.19
|
|
|
Biochemical Characterization of Human Foamy Virus Integrase
|
|
강승이/Kang SY
오수아/이학성/한성태/서진욱/신차균/Oh SA/Lee HS/Han ST/Seo JW/Shin CG
|
|
|
Abstract
|
|
|
|
A bacterial expression vector for the human foamy virus (HFV) integrase was constructed and expressed In Escherichia coli. By two-step purification using a nickel-chela ted column and a SP-sepharose chromatography, the HFV integrase protein of 43 kDa was purified to near homogeneity, and used to investigate biochemical characteristics of the enzymatic activities, such as endonucleolytic and disintegration activities. 0ligonucleotide substrates were specifically and efficiently cleaved by the purified HFV integrase in the presence of Mn+2, but not in the presence of Mg+2, indicating that the HFV integrase is not able to use Mg+2 as a cofactor. Endonucleolytic reaction was almost completed in 60 min at 37 ℃. In addition, the maximum enzymatic activities were observed at 5 mM Mn+2 in the buffer of which pH was from 7.0 to 9.0. The endonucleolytic activities were dose-dependently blocked in the addition of baicalein or chicolic acid which is a well-known inhibitor of human immunodeficiency virus integrase.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|